selenite duplex table

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    Figure 1. Selenite causes apoptosis with superoxide accumulation in wt p53–expressing LNCaP cells. A, dosedependent effect of selenite on cell viability as shown by the MTT assay. Cells were treated with 0 to μmol/L selenite for 5 days.

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    In addition, selenite at low concentrations activated other antiapoptotic pathways, such as AKT and ERK, whereas high concentrations of selenite induced activation of p53 and oxidative stress, which mediated the antitumor activity of selenite by causing mitochondrial dysfunction and caspase activation.

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  • SUPPLEMENTARY MATERIALS Table S1. Materials Used for …

    selenite media supplement SigmaAldrich, St. Louis, MO 5 µg/ml insulin, 5 ... Table S3. Antibodies Used for Immunfluorescent Staining and Western Blot ... Table S4. Oligonucleotide siRNA duplex Used for Notch1 Knockdown Names Sequence (5` to 3`) Notch1siRNA (A) CAACUACUACCUCUGCUUAdTdT ...

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    Experimental deformation of a singlelayer … A new deformation apparatus has been used to model the internal kinematics of salt diapirs. Composite natural samples consisting of a single layer of anhydrite,

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    Disruption of redox homeostasis is a prominent feature in the pathogenesis of Huntington's disease (HD). Selenium an essential element nutrient that modulates redox pathways and has been reported to provide protection against both acute neurotoxicity ( methamphetamine) and chronic neurodegeneration ( tauopathy) in mice.

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  • efficiency of Escherichia coli selenocysteine insertion is ...

    Although the addition of sodium selenite to the growth medium did not affect phage production, it did increase the level of Sec insertion, as shown by the chemical reactivity of the resulting phage. ... gel purified and annealed to the duplex extension primer. The duplex was extended with dNTPs and Klenow fragment, digested with Acc65I and EagI ...

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  • The efficiency of Escherichia coli selenocysteine ...

    Feb 01, 2000· The sequences of selected clones are shown in Table Table1. 1. Although the immediate downstream nucleotide was fully randomized in the TGAN library insert, the majority (74%) of the resulting phage clones possessed a downstream purine.

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  • Sodium SeleniteInduced Apoptosis in Murine BLymphoma ...

    The effects of sodium selenite on cell viability and proliferation. (A) A20 B lymphoma cells at a density of 1 × 10 5 cells/ml were incubated with media containing 0, , 5, 25, or 50 μM sodium selenite. At 24, 72, and 96 h, the number of viable cells was determined by Trypan blue exclusion.

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    In Courteney Cox's master bedroom designed by Trip Haenisch, the custommade head and footboards are zinc, the selenite bedside tables are by Blackman Cruz, and the bedding is by Deborah Sharpe Linens; the twotone Swag Leg chair is by Courteney Cox

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  • Sodium selenite induces apoptosis by ROS ... SpringerLink

    Materials and methods. NB4 cells were treated with 20 μM sodium selenite for different times. The activation of caspases and ER stress markers, ROS levels, mitochondrial membrane potential and cell apoptosis induced by sodium selenite were analyzed by immunoblotting analysis, DCF fluorescence and flow cytometric respectively. siRNA was used to detect the effect of GADD153 on seleniteinduced ...

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  • Sodium Selenite Induces SuperoxideMediated Mitochondrial ...

    Abstract. Malignant gliomas are resistant to various proapoptotic therapies, such as radiotherapy and conventional chemotherapy. In this study, we show that selenite is preferentially cytotoxic to various human glioma cells over normal astrocytes via autophagic cell death.

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    Table 1 – Design Bioreactor Influent Criteria Following the settling pond, water is sent to a series of fixedfilm, biological reactors. The bioreactors utilize a mixture of specifically selected, naturally occurring bacteria. ... range, selenate and/or selenite is then

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